Rodrigues dos Santos, J. A.; Amorim, T.; Nunes, D.; Couto, A.; Miranda, F. (2013). Alterations induced by two different soccer workouts in CK, Cortisol and Testosterone serum levels. Journal of Sport and Health Research. 5(2):149-156.



The purpose of the study was to examine the effects of two different soccer workouts on basal values of cortisol, testosterone and creatine kinase (CK) in professional soccer players one day after exertion.

Twenty three professional soccer players (age: 25.2±3.1 years) realised two different workouts (W1 and W2). Twenty-two hours after the workouts blood samples were collected in fasting state from the antecubital vein for verifying changes of the selected biomarkers.

Cortisol increased significantly from 278.8±19.8 nmol.l-1 (average basal values out-season) to 527.8±76.4 nmol.l-1 (p<0.001) and 747.7±121.1 nmol.l-1 (p<0.0001) after W1 and W2, respectively. Testosterone was not significantly (p>0.05) altered [19.8±7.2 nmol.l-1 (out-season); 24.4±5.6 nmol.l-1 after W1; 23.4±6.1 nmol.l-1 after W2]. Creatine Kinase (CK) increased significantly from 101.5±30.2 U/L (out-season) to 178.4±88.5 U/L (p<0.01) after W1 and 419.8±372.0 U/L (p<0.0001) after W2.

It was concluded that W2 induced a more significant increase in CK and cortisol serum levels. These increases are probably due to the relative higher number of eccentric contractions in W2. 


Hormones; Enzymes; Training control    


Journal of Sport and Health Research - 2009